Methods for preparing stable deoxygenated peg-hemoglobin conjugate solutions comprising an antioxidant
AssigneeSangart, Inc.
InventorsKim D. VANDEGRIFF, Ashok S. Malavalli
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Abstract
The present invention is a method for preparing stable HBOC solutions. Specifically, the method comprises the steps of deoxygenating a PEG-Hb conjugate and adding one or more antioxidants during or following the deoxygenating step to form a stabilized PEG-Hb conjugate. The Hb in the PEG-Hb conjugate is not crosslinked and the stabilized PEG-Hb conjugate has a p50 less than that of native SFH from the same animal source when measured under the same conditions. Specifically, the p50 is 6±2 mmHg or less than 10 mmHg.
Claims14
A method of stabilizing a polyethylene glycol hemoglobin conjugate (PEG-Hb) comprising the steps of:
- a) deoxygenating the PEG-Hb conjugate;
- and
- b) adding one or more antioxidants during or following the deoxygenating of PEG-Hb conjugate to form a stabilized PEG-Hb conjugate;
- wherein the hemoglobin in the PEG-Hb conjugate is not crosslinked and
- wherein the stabilized PEG-Hb conjugate has a p50 less than that of native stroma free hemoglobin from the same animal source when measured under the same conditions.
Dependencies are inferred from the claim text.