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AssigneesNational University Corporation Hokkaido University, Fuso Pharmaceutical Industries, Ltd.
InventorsYusuke Suzuki, Hiroshi Tahara, Keiichi Yamamoto, Yuzuru Kitahara, Yasuhiko Suzuki
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As a result of dedicated research, the present inventors have successfully invented a collagen gene construct which can be easily purified and maintains a triple helix structure equivalent to that of naturally-occurring collagen while having a low molecular weight. Specifically, one-step purification by affinity purification is enabled because CR-D (a signal peptide) has a carbohydrate recognition domain. By substituting a portion of a human collagen structural gene of the present invention with the collagen-like structural gene portion of MBL, a low-molecular-weight collagen which maintains a triple helix structure and is thermally stable can be obtained with high purity and in large quantities.
A recombinant protein having a triple helix structure, which comprises a protein encoded by a polynucleotide comprising (i) to (v) below in order from the amino terminus:
A method for producing a protein having a triple helix structure, wherein the method comprises the steps of:
Dependencies are inferred from the claim text.