# US 2012/0059150 A1 · Methods for the production of apolipoproteins in transgenic plants

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- Number: US 2012/0059150 A1
- Document ID: US20120059150A1
- Status: Published application
- Kind: A1
- Application number: 12/819,068
- Filed: 2010-06-18
- Published: 2012-03-08
- Earliest claimed: 2003-11-14
- Assignees: SemBioSys Genetics Inc.
- Inventors: Maurice M. MOLONEY; Alexandra Reid
- Web page: https://patentgrep.com/patent/US20120059150A1

## CPC

- C07K 14/775: Apolipopeptides
- A61P 3/06: Antihyperlipidemics
- C12N 15/8257: {for the production of primary gene products, e.g. pharmaceutical products, interferon}
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

Methods for the production of an apolipoprotein in plants are described. In one embodiment, the present invention provides a method for the expression of apolipoprotein in plants comprising: (a) providing a chimeric nucleic acid construct comprising in the 5′ to 3′ direction of transcription as operably linked components: (i) a nucleic acid sequence capable of controlling expression in plant cells; and (ii) a nucleic acid sequence encoding an apolipoprotein polypeptide; (b) introducing the chimeric nucleic acid construct into a plant cell; and growing the plant cell into a mature plant capable of setting seed wherein the seed expresses apolipoprotein.

## Claims

### Claim at position 1

1-38. (canceled)

### Claim 39

A composition comprising substantially pure oil bodies comprising apolipoprotein obtained from plants.

### Claim 40

A nucleic acid sequence encoding apolipoprotein linked to nucleic acid sequence comprising a nucleic acid capable of controlling expression in a plant cell.

### Claim 41 (depends on 40)

A nucleic acid sequence according to claim 40 wherein said plant cell is a seed cell.

### Claim 42 (depends on 41)

A nucleic acid sequence according to claim 41 wherein said nucleic acid sequence capable of controlling expression in a plant cell is a seed-preferred promoter.

### Claim 43 (depends on 42)

A nucleic acid according to claim 42 wherein said seed-preferred promoter is the phaseolin promoter.

### Claim 44 (depends on 41)

A nucleic acid sequence according to claim 41 wherein said nucleic acid sequence capable of controlling expression in plant seeds is a constitutive promoter.

### Claim 45 (depends on 44)

A nucleic acid sequence according to claim 44 wherein said promoter is the ubiquitin promoter.

### Claim 46

A recombinant expression vector suitable for expression in a plant cell comprising a nucleic acid sequence encoding an apolipoprotein polypeptide.

### Claim 47

A method for preparing substantially pure apolipoprotein comprising: (a) providing a chimeric nucleic acid construct comprising in the 5′ to 3′ direction of transcription as operably linked components: (i) a nucleic acid sequence capable of controlling expression in plant seed cells; and (ii) a nucleic acid sequence encoding an apolipoprotein polypeptide; (b) introducing the chimeric nucleic acid construct into a plant cell; (c) growing the plant cell into a mature plant; and (d) obtaining seed from said plant wherein the seed comprises apolipoprotein; and (e) separating apolipoprotein from the plant seed constituents to obtain substantially pure apolipoprotein.

### Claim 48

(canceled)

### Claim 49

A composition comprising substantially pure apolipoprotein obtained from a plant.