Methods of using oligomeric compounds comprising 2'-substituted nucleosides
AssigneeIsis Pharmaceuticals, Inc.
InventorsThazha P. Prakash, Andrew M. Siwkowski, Eric E. Swayze, Balkrishen Bhat
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Abstract
The present disclosure provides oligomeric compounds comprising at least one 2′-fluoroethoxy modified nucleoside of formula I and methods of using these oligomeric compounds. The methods provided herein include contacting a cell or administering to an animal at least one of the oligomeric compounds. In certain embodiments, the oligomeric compounds hybridize to a portion of a target RNA resulting in loss of normal function of the target RNA.
Claims38
A method comprising contacting a cell with an oligomeric compound, wherein said oligomeric compound comprises at least one 2′-fluoroethoxy modified nucleoside having formula I:
- wherein independently for each of said 2′-fluoroethoxy modified nucleosides having formula I:
- Bx is a heterocyclic base moiety;
- one of T1 and T2 is an internucleoside linking group attaching the 2′-fluoroethoxy modified nucleoside of formula I to the oligomeric compound and the other of T1 and T2 is H, a hydroxyl protecting group, a 5′ or 3′-terminal group or an internucleoside linking group attaching the 2′-fluoroethoxy modified nucleoside of formula I to the oligomeric compound;
- R1 and R2 are each independently H or F;
- and
- wherein said oligomeric compound comprises from about 8 to about 40 linked monomeric subunits and is complementary to at least a portion of a target RNA.
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Dependencies are inferred from the claim text.