Back to results
US 2012/0058920 A1Published application
Method for Identifying Inhibitors Against Dengue Virus
OwnerEdwin Yunhao Gong, Tania Pauline Eduard Ivens +3inventors
InventorsEdwin Yunhao Gong, Tania Pauline Eduard Ivens, Marleen Clynhens, Pedro Miguel Jales Lory, Guenter Kraus
Life of this patent
Estimating the term…
Abstract
The present invention concerns a method for identifying inhibitors against dengue virus subtypes 1, 2, 3 or 4 and its use in a high throughput mode.
Claims8
Method for identifying inhibitors against dengue viruses serotypes 1, 2, 3 or 4 by screening chemical compounds or chemical compound libraries comprising:
- a) bringing about 1000 to 2000 Vero cells or Huh 7.5 cells into contact with a chemical compound,
- b) adding dengue virus to said cells and chemical compound of step a),
- c) incubating said cells, chemical compound and virus of step b) at 37° C. until viral cytopathic effect (CPE) in the virus control reaches about 100%,
- d) adding luciferase enzyme and luciferase substrate to the cells, compound, virus,
- e) measuring the luminescence and calculating the EC50 value, wherein the EC50 value correlates with the inhibitory activity of the compound against dengue virus.
2 more elements
Dependencies are inferred from the claim text.