Universal Tags, Probes and Detection Methods For Multiple Targets Detection of Biomolecules

A multiple targets detection method of biomolecules, characterized in that the method comprises steps of:

• 1) preparing a universal tag;
• 2) preparing a probe;
• 3) linking the probe to a modified solid phase support to form probe arrays;
• 4) dissolving the universal tag and samples to be tested into a hybridization solution to hybridize with the probe arrays;
• or, hybridizing the sample to be tested with the probe first, then hybridizing the universal tag with the probe arrays after rinsing;
• 5) rinsing to remove the redundant samples and the redundant universal tag;
• and
• 6) detecting and analyzing hybridization signal,
• wherein,
• the universal tag is a fragment of DNA, RNA, peptide nucleic acid, or LNA, and is 3-20 mer in length;
• the probe contains in order from 3′ terminus to 5′ terminus, a nucleotide sequence which is reverse complementary to a target molecule or a portion of a target molecule, and a nucleotide sequence which is reverse complementary to the universal tag, and a fragment of poly (T) or poly (A) optionally added on the 3′ terminus;
• or the probe contains in order from 3′ terminus to 5′ terminus, a nucleotide sequence which is reverse complementary to the universal tag, a nucleotide sequence which is reverse complementary to a target molecule or a portion of a target molecule, and a fragment of poly (T) or poly (A) optionally added on the 5′ terminus.