Reprogramming immortalized b cells
AssigneeCellular Dynamics International, Inc.
InventorsJAMES THOMSON, Deepika Rajesh, Sarah Jane Dickerson, Amanda Mack, Michael Miller
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Abstract
Methods and composition for providing induced pluripotent stem (iPS) cells are provided. For example, in certain aspects methods including reprogramming B lymphocytes transformed by episomal vectors such as Epstein-Barr virus-based vectors are described. Furthermore, the invention provides induced pluripotent stem cells essentially free of exogenous elements and having B cell immunoglobin variable region rearrangement.
Claims22
A method for producing induced pluripotent stem cells (iPS cells) from immortalized B cells, wherein the method comprises:
- a) obtaining immortalized B cells that have been immortalized by virtue of one or more immortalizing Epstein-Barr virus (EBV) elements;
- and
- b) reprogramming the immortalized B cells by effecting expression of exogenous reprogramming factors therein, thereby producing iPS cells.
A human iPS cell having a genome that comprises an incomplete set of B cell immunoglobin variable region genes compared with an embryonic stem cell, wherein the iPS cell is essentially free of exogenous genetic elements.
Dependencies are inferred from the claim text.