Methods and compositions for stem cell cultures
AssigneeThe Regents of the University of California
InventorNoboru Sato
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Abstract
The disclosure provides methods and compositions useful for culturing stem cell including embryonic stem cells, adult stem cells, and embryonic germ cells.
Claims35
A composition comprising a basal medium and a myosin II inhibitor.
(canceled)
A composition comprising basal medium supplemented with non-essential amino acids, an anti-oxidant, a reducing agent, growth factors, a pyruvate salt and a myosin II inhibitor.
A kit comprising poly-D-lysine, a defined culture medium and a myosin II inhibitor.
A method of culturing stem cells, comprising:
- suspending the stem cells in a culture medium comprising a myosin II inhibitor;
- and
- culturing the stem cells in the presence of a poly-D-lysine coated tissue culture substrate.
A kit of comprising a myosin II inhibitor compartmentalized to be added to a basal medium or a defined medium.
A composition comprising a defined culture medium comprising recombinant bFGF, recombinant TGFβ wherein the composition is serum-free and comprises a myosin II inhibitor.
A method of culturing stem cell in an animal free medium comprising a poly-D-lysine substrate and a medium that promotes proliferation and growth of stem cells, wherein the medium comprises animal free components and a myosin II inhibitor, a ROCK inhibitor or a combination thereof.
Dependencies are inferred from the claim text.