Mineralized Three-Dimensional Bone Constructs
OwnerMark S.F. Clarke, Alamelu Sundaresan +1inventors
InventorsMark S.F. Clarke, Alamelu Sundaresan, Neal R. Pellis
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Abstract
The present disclosure provides ex-vivo derived mineralized three-dimensional bone constructs. The bone constructs are obtained by culturing osteoblasts and osteclast precursors under randomized gravity vector conditions. Preferably, the randomized gravity vector conditions are obtained using a low shear stress rotating bioreactor, such as a High Aspect Ratio Vessel (HARV) culture system. The bone constructs of the disclosure have utility in physiological studies of bone formation and bone function, in drug discovery, and in orthopedics.
Claims13
A method for producing mineralized three-dimensional bone constructs, the method comprising:
- culturing osteoclast precursors and osteoblasts in a matrix-free culture medium using a low shear rotational three-dimensional tissue culture technique under conditions effective to form aggregates comprising said osteoblasts and said osteoclast precursors, and culturing said aggregates using said low shear rotational three-dimensional tissue culture technique in a matrix-free mineralization culture medium that promotes mineralization of said aggregates.
A method for producing mineralized three-dimensional bone constructs, the method comprising:
- (a) introducing osteoclast precursors and osteoblasts into a cylindrical culture vessel that rotates about a central horizontal axis, said cylindrical culture vessel comprising a matrix-free culture medium;
- (b) culturing said osteoblasts and said osteoclast precursors in said cylindrical culture vessel during horizontal rotation at a rate effective to create low shear conditions and to promote the formation of aggregates comprising said osteoclast precursors and said osteoblasts;
- and
- (c) introducing a matrix-free mineralization culture medium into said cylindrical culture vessel and culturing said aggregates during horizontal rotation at a rate effective to create low shear conditions, wherein said mineralized three-dimensional bone constructs are formed.
Dependencies are inferred from the claim text.