High Surface Area Substrate for Cell Culture
AssigneeCorning Incorporated
InventorsMichelle Dawn Fabian, Timothy Edward Myers, Kyle Patrick Snyder, Florence Verrier
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Abstract
A cell culture microcarrier includes (1) a polystyrene microcarrier base having a remnant of a carboxylic acid group, and (ii) a polypeptide conjugated to the base via the remnant of the carboxylic acid group. The polypeptide may contain a cell adhesive sequence, such as RGD. Cells cultured with such microcarriers exhibit peptide-specific binding to the microcarriers.
Claims18
A microcarrier for cell culture, comprising:
- a polystyrene microcarrier base having residues of carboxylic acid functional groups;
- and
- a polypeptide conjugated to the base via the residue of the carboxylic acid functional group.
A microcarrier for cell culture, consisting essentially of:
- a polystyrene microcarrier base having one or both of (i) a pendant carboxylic acid group, and (ii) one or more pendant amides formed from reaction of carboxylic acid groups with one or more monoamine;
- and
- one or more polypeptides conjugated to the base via an amide bond formed from the reaction of an amine of the polypeptide and pendant carboxylic acid group of the microcarrier base.
A method of making a microcarrier for cell culture, comprising:
- providing a polystyrene microcarrier base having pendant carboxylic acid functional groups;
- and
- conjugating a polypeptide to a carboxylic acid group of a microcarrier base to form the microcarrier.
A method for culturing cells, comprising:
- contacting cells with a cell culture medium having microcarriers, wherein the microcarrier comprises
- a polystyrene microcarrier base having residues of carboxylic acid functional groups;
- and
- a polypeptide conjugated to the base via the residue of the carboxylic acid functional group;
- and
- culturing the cells in the medium.
Dependencies are inferred from the claim text.