Synthetic Polysaccharide Microcarriers for Culturing Cells
AssigneeCorning Incorporated
InventorsSophie Deshayes, David Henry, Martial Hervy
Life of this patent
Estimating the term…
Abstract
A cell culture polysaccharide microcarrier includes (1) a cross-linked polysaccharide microcarrier base having a neutral or negative charge at pH 7, and (ii) a polypeptide conjugated to the base. The polypeptide may contain a cell adhesive sequence, such as RGD. Cells cultured with such microcarriers exhibit peptide-specific binding to the microcarriers.
Claims20
A microcarrier for cell culture, comprising:
- a cross-linked polysaccharide hydrogel microcarrier base having a neutral or negative charge at pH 7;
- and
- a polypeptide conjugated to the base.
A method for forming a cell culture microcarrier, comprising:
- providing a net negatively or neutral charged polysaccharide hydrogel microcarrier base having pendant carboxylic acid functional groups;
- and
- conjugating a synthetic polypeptide to a carboxylic acid group of the microcarrier base to form the microcarrer.
A method for culturing cells, comprising:
- contacting cells with a cell culture medium having microcarriers, wherein the microcarrier comprises
- (i) a neutral or negatively charged polysaccharide hydrogel microcarrier base, and
- (ii) a polypeptide conjugated to the base, wherein the polypeptide comprises and RGD amino acid sequence;
- and
- culturing the cells in the medium.
The method of clam 19, wherein the cells are stem cells and the medium is a chemically defined medium.
Dependencies are inferred from the claim text.