Biofuel production in prokaryotes and eukaryotes
AssigneeSapphire Energy, Inc.
InventorsNicole A. Heaps, Craig A. Behnke, David Molina
Life of this patent
Estimating the term…
Abstract
Terpene synthases are enzymes that directly convert IPP & DMAPP to terpenes, such as fusicoccadiene. Described herein are methods and compositions for the production of terpenes and terpenoids for use as fuel molecules or other useful components. Genetically engineered enzymes capable of producing terpenes and terpenoids are also described.
Claims21
1-248. (canceled)
An isolated polynucleotide capable of transforming a photosynthetic bacterium, a yeast, an alga, or a vascular plant, wherein the polynucleotide comprises a nucleic acid sequence of SEQ ID NO:4, SEQ ID NO:
- 7, SEQ ID NO:
- 39, SEQ ID NO:
- 1, SEQ ID NO:
- 9, SEQ ID NO:
- 11, SEQ ID NO:
- 15, SEQ ID NO:
- 17, SEQ ID NO:
- 21, SEQ ID NO:
- 26, SEQ ID NO:
- 28, SEQ ID NO:
- 32, SEQ ID NO:
- 34, SEQ ID NO:
- 37, SEQ ID NO:
- 44, SEQ ID NO:
- 46, SEQ ID NO:
- 49, SEQ ID NO:
- 51, SEQ ID NO:
- 54, or SEQ ID NO:
- 56;
- or a nucleic acid sequence comprising at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity to a nucleic acid sequence of SEQ ID NO:4, SEQ ID NO:
- 7, SEQ ID NO:
- 39, SEQ ID NO:
- 1, SEQ ID NO:
- 9, SEQ ID NO:
- 11, SEQ ID NO:
- 15, SEQ ID NO:
- 17, SEQ ID NO:
- 21, SEQ ID NO:
- 26, SEQ ID NO:
- 28, SEQ ID NO:
- 32, SEQ ID NO:
- 34, SEQ ID NO:
- 37, SEQ ID NO:
- 44, SEQ ID NO:
- 46, SEQ ID NO:
- 49, SEQ ID NO:
- 51, SEQ ID NO:
- 54, or SEQ ID NO:
- 56.
An isolated polynucleotide capable of transforming a photosynthetic bacterium, a yeast, an alga, or a vascular plant, wherein the polynucleotide encodes for a protein comprising an amino acid sequence of SEQ ID NO:
- 2, SEQ ID NO:
- 38, SEQ ID NO:
- 10, SEQ ID NO:
- 16, SEQ ID NO:
- 22, SEQ ID NO:
- 27, SEQ ID NO:
- 33, SEQ ID NO:
- 45, SEQ ID NO:
- 50, or SEQ ID NO:
- 55;
- or an amino acid sequence that has at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity to the amino acid sequence of SEQ ID NO:
- 2, SEQ ID NO:
- 38, SEQ ID NO:
- 10, SEQ ID NO:
- 16, SEQ ID NO:
- 22, SEQ ID NO:
- 27, SEQ ID NO:
- 33, SEQ ID NO:
- 45, SEQ ID NO:
- 50, or SEQ ID NO:
- 55.
A method of expressing a terpene synthase or a portion of a terpene synthase capable of modulating an isoprenoid pathway in a photosynthetic bacterium, a yeast, an alga, or a vascular plant comprising:
- a) transforming the photosynthetic bacterium, yeast, alga, or vascular plant with an exogenous polynucleotide sequence comprising a nucleotide sequence encoding for the terpene synthase or portion of the terpene synthase;
- and
- b) expressing the terpene synthase or portion of the terpene synthase.
A photosynthetic bacterium, yeast, alga, or vascular plant transformed with an exogenous polynucleotide sequence encoding an enzyme that modulates an isoprenoid pathway of the photosynthetic bacterium, yeast, alga, or vascular plant.
Dependencies are inferred from the claim text.