Loading patent…
AssigneesCalifornia Institute of Technology, Gevo, Inc.
InventorsThomas Buelter, Peter Meinhold, Reid M. Renny Feldman, Andrew Hawkins, Sabine Bastian, Frances Arnold, Jun Urano
Estimating the term…
The present invention is generally provides recombinant microorganisms comprising engineered metabolic pathways capable of producing C3-C5 alcohols under aerobic and anaerobic conditions. The invention further provides ketol-acid reductoisomerase enzymes which have been mutated or modified to increase their NADH-dependent activity or to switch the cofactor preference from NADPH to NADH and are expressed in the modified microorganisms. In addition, the invention provides isobutyraldehyde dehydrogenase enzymes expressed in modified microorganisms. Also provided are methods of producing beneficial metabolites under aerobic and anaerobic conditions by contacting a suitable substrate with the modified microorganisms of the present invention.
An isolated nucleic acid molecule encoding a modified ketol-acid reductoisomerase (KARI), wherein said modified KARI is a polypeptide which has been engineered to comprise two amino acid substitutions that alter the cofactor usage of the polypeptide, wherein said two amino acid substitutions correspond to amino acid residues Arg76 and Ser78 of the E. coli ketol-acid reductoisomerase (SEQ ID NO:
Dependencies are inferred from the claim text.