Cal a-related acyltransferases and methods of use, thereof
AssigneeDanisco US Inc.
InventorSusan Madrid
Life of this patent
Estimating the term…
Abstract
Compositions and methods relating to lipase/acyltransferase enzymes identified in prokaryotes and eukaryotes are described. These enzymes can be used in such applications as lipid stain removal from fabrics and hard surfaces and chemical synthesis reactions.
Claims36
A recombinant lipase/acyltransferase enzyme having only limited amino acid sequence identity to Candida albicans Cal-L lipase/acyltransferase, comprising:
- a) a first amino acid sequence motif GX1SX2G at residues corresponding to positions 192-196 of the Cpa-L amino acid sequence (SEQ ID NO:
- 8), where X1 is an aromatic amino acid and X2 is an amino acid selected from the group consisting of G, E, or Q;
- b) a second amino acid sequence motif YAX1X2X3, at residues corresponding to positions 210-214 of the Cpa-L amino acid sequence (SEQ ID NO:
- 8), where X1 is P or K, X2 is an acidic amino acid, and X3 is a non-polar aliphatic amino acid;
- c) lipase/esterase activity based on hydrolysis of p-nitrophenylbutyrate in an aqueous solution.
A recombinant lipase/acyltransferase enzyme having at least 90% amino acid sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:
- 2, SEQ ID NO:
- 11, SEQ ID NO:
- 14, SEQ ID NO:
- 17, SEQ ID NO:
- 20, SEQ ID NO:
- 23, SEQ ID NO:
- 26, SEQ ID NO:
- 29, SEQ ID NO:
- 32, SEQ ID NO:
- 35, SEQ ID NO:
- 38, SEQ ID NO:
- 41, SEQ ID NO:
- 44, SEQ ID NO:
- 47, SEQ ID NO:
- 50, and SEQ ID NO:
- 53.
A composition comprising a recombinant lipase/acyltransferase enzyme having at least 90% amino acid sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:
- 2, SEQ ID NO:
- 11, SEQ ID NO:
- 14, SEQ ID NO:
- 17, SEQ ID NO:
- 20, SEQ ID NO:
- 23, SEQ ID NO:
- 26, SEQ ID NO:
- 29, SEQ ID NO:
- 32, SEQ ID NO:
- 35, SEQ ID NO:
- 38, SEQ ID NO:
- 41, SEQ ID NO:
- 44, SEQ ID NO:
- 47, SEQ ID NO:
- 50, and SEQ ID NO:
- 53.
(canceled)
An expression vector comprising a polynucleotide encoding the lipase/acyltransferase enzyme Cal-L or Cpa-L and a signal sequence to cause secretion of the lipase/acyltransferase enzyme.
Dependencies are inferred from the claim text.