Method of expressing foreign protein in plastids
AssigneesVanga Siva Reddy, Sadhu Leelavathi, Amit Bhardwaj
InventorsVanga Siva Reddy, Sadhu Leelavathi, Amit Bhardwaj
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Abstract
The present invention relates to a method of expressing a foreign protein in the plastid of a host cell and secreting said protein into the cytoplasm of the host cell comprising the steps of making a construct of vector linked to a coding sequence of the fusion protein comprising of signal peptide sequence followed by in-frame fusion to a foreign gene; and stably integrating said construct into the plastid genome. The present invention also relates to a method of targeting the expressed and secreted proteins from the plastids to the nucleus of the host cell. The present invention further relates to the method where the host cell is of any higher plant or any organism including single cell algae.
Claims26
A method of expressing a foreign protein in a plastid of a host cell and secreting said protein into cytoplasm of the host cell comprising the steps of
- (a) making a construct of vector linked to a coding sequence of a fusion protein comprising a signal peptide sequence followed by in-frame fusion to a foreign gene;
- and
- (b) stably integrating said construct of step (a) into the plastid genome.
A method of expressing a foreign protein in a plastid of a host cell, secreting said protein into the cytoplasm of the host cell and targeting said protein to the nucleus of the host cell comprising the steps of
- (a) making a construct of vector linked to a coding sequence of the fusion protein comprising a signal peptide sequence followed by in-frame fusion to a nuclear localization signal followed by a foreign gene;
- and
- (b) stably integrating said construct of step (a) into the plastid genome.
A method of expressing a foreign protein in a plastid of a host cell selected from the group comprising a higher plant or organism including single cell algae;
- and secreting said protein into cytoplasm of the host cell comprising the steps of making a construct of pVSR 326 plasmid vector linked to a coding sequence of the fusion protein comprising a signal peptide sequence selected from the group comprising a signal peptide of xylanase or any prokaryotic signal peptide having similar function, followed by in-frame fusion to a foreign gene selected from the group comprising a Green Fluorescent Protein or any other protein;
- and stably integrating said construct into the plastid genome.
A method of expressing a foreign protein in a plastid of a host cell selected from the group comprising any higher plant or organism;
- secreting said protein into the cytoplasm of the host cell and targeting said protein to the nucleus of the host cell comprising the steps of making a construct of pVSR 326 plasmid vector linked to a coding sequence of the fusion protein comprising signal peptide sequence selected from the group comprising signal peptide of xylanase or any prokaryotic signal peptide having similar function, followed by in-frame fusion to a nuclear localization signal from SV 40 antigen, followed by a foreign gene selected from group comprising Green Fluorescent Protein or any other protein;
- and stably integrating said construct into the plastid genome.
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Dependencies are inferred from the claim text.