Mutant microorganism and method for producing peptide using the same

16:

• A mutant Escherichia coli, comprising at least one disrupted gene, wherein the gene is selected from the group consisting of a gene encoding aminoacylhistidine peptidase, a gene encoding leucylaminopeptidase and a gene encoding isoaspartyldipeptidase, respectively on a chromosome;
• and
• wherein said Escherichia coli is transformed with a recombinant DNA comprising a polynucleotide selected from the group consisting of
• a polynucleotide which hybridizes under a stringent condition with a complement consisting of the nucleotide sequence complementary to nucleotide residues 61-1908 of the nucleotide sequence of SEQ ID NO:
• 13,
• a polynucleotide which hybridizes under a stringent condition with a complement consisting of the nucleotide sequence complementary to nucleotide residues 127-1908 of the nucleotide sequence of SEQ ID NO:
• 13,
• a polynucleotide which hybridizes under a stringent condition with a complement consisting of the nucleotide sequence complementary to nucleotide residues 61-1917 of the nucleotide sequence of SEQ ID NO:
• 19,
• a polynucleotide which hybridizes under a stringent condition with a complement consisting of the nucleotide sequence complementary to nucleotide residues 121-1917 of the nucleotide sequence of SEQ ID NO:
• 19, and
• a polynucleotide encoding a protein selected from the group consisting of
• a protein comprising the amino acid sequence of SEQ ID NO:
• 20,
• a protein consisting of an amino acid sequence including substitution, deletion, insertion, and/or addition of a total of one to thirty amino acid residues in the amino acid sequence of SEQ ID NO:
• 14,
• a protein consisting of an amino acid sequence including substitution, deletion, insertion, and/or addition of a total of one to thirty amino acid residues in the amino acid sequence of SEQ ID NO:
• 20,
• a protein comprising the amino acid sequence consisting of amino acid residues 23-616 of the amino acid sequence of SEQ ID NO:
• 14, and
• a protein consisting of an amino acid sequence including substitution, deletion, insertion, and/or addition of a total of one to thirty amino acid residues in the amino acid sequence consisting of amino acid residues 21-619 of the amino acid sequence of SEQ ID NO:
• 20.

The mutant Escherichia coli according to claim 16, wherein the polynucleotide is selected from the group consisting of

• A polynucleotide which hybridizes under a stringent condition with a complement consisting of the nucleotide sequence complementary to nucleotide residues 61-1908 of the nucleotide sequence of SEQ ID NO:
• 13,
• a polynucleotide which hybridizes under a stringent condition with a complement consisting of the nucleotide sequence complementary to nucleotide residues 127-1908 of the nucleotide sequence of SEQ ID NO:
• 13,
• a polynucleotide which hybridizes under a stringent condition with a complement consisting of the nucleotide sequence complementary to nucleotide residues 61-1917 of the nucleotide sequence of SEQ ID NO:
• 19,
• a polynucleotide which hybridizes under a stringent condition with a complement consisting of the nucleotide sequence complementary to nucleotide residues 121-1917 of the nucleotide sequence of SEQ ID NO:
• 19.

The Escherichia coli according to claim 16, wherein the protein is selected from the group consisting of

• a protein consisting of an amino acid sequence including substitution, deletion, insertion, and/or addition of a total of one to thirty amino acid residues in the amino acid sequence of SEQ ID NO:
• 14, and
• a protein consisting of an amino acid sequence including substitution, deletion, insertion, and/or addition of a total of one to thirty amino acid residues in the amino acid sequence consisting of amino acid residues 23-616 of the amino acid sequence of SEQ ID NO:
• 14.

The mutant Escherichia coli according to claim 16, wherein the protein is selected from the group consisting of

• a protein consisting of an amino acid sequence including substitution, deletion, insertion, and/or addition of a total of one to thirty amino acid residues in the amino acid sequence of SEQ ID NO:
• 20, and
• a protein consisting of an amino acid sequence including substitution, deletion, insertion, and/or addition of a total of one to thirty amino acid residues in the amino acid sequence consisting of amino acid residues 21-619 of the amino acid sequence of SEQ ID NO:
• 20.

The mutant Escherichia coli according to claim 16, wherein said Escherichia coli is transformed with a recombinant DNA comprising a polynucleotide encoding a protein selected from the group consisting of

• a protein consisting of an amino acid sequence including substitution, deletion, insertion, and/or addition of a total of one to thirty amino acid residues in the amino acid sequence of SEQ ID NO:
• 14 and having 50% or more of the enzymatic activity of the amino acid sequence of SEQ ID NO:
• 14 at 50° C. pH 8,
• a protein consisting of an amino acid sequence including substitution, deletion, insertion, and/or addition of a total of one to thirty amino acid residues in the amino acid sequence of SEQ ID NO:
• 20 and having 50% or more of the enzymatic activity of the amino acid sequence of SEQ ID NO:
• 20 at 50° C. pH 8,
• a protein consisting of an amino acid sequence including substitution, deletion, insertion, and/or addition of a total of one to thirty amino acid residues in the amino acid sequence consisting of amino acid residues 23-616 of the amino acid sequence of SEQ ID NO:
• 14 and having 50% or more of the enzymatic activity of amino acid residues 23-616 of the amino acid sequence of SEQ ID NO:
• 14 at 50° C. pH 8, and
• a protein consisting of an amino acid sequence including substitution, deletion, insertion, and/or addition of a total of one to thirty amino acid residues in the amino acid sequence consisting of amino acid residues 21-619 of the amino acid sequence of SEQ ID NO:
• 20 and having 50% or more of the enzymatic activity of amino acid residues 21-619 of the amino acid sequence of SEQ ID NO:
• 20 at 50° C. pH 8.

The mutant Escherichia coli according to claim 16, wherein said Escherichia coli is transformed with a recombinant DNA comprising a polynucleotide encoding a protein selected from the group consisting of

• a protein consisting of an amino acid sequence including substitution, deletion, insertion, and/or addition of a total of one to thirty amino acid residues in the amino acid sequence of SEQ ID NO:
• 14 and having 80% or more of the enzymatic activity of the amino acid sequence of SEQ ID NO:
• 14 at 50° C. pH 8,
• a protein consisting of an amino acid sequence including substitution, deletion, insertion, and/or addition of a total of one to thirty amino acid residues in the amino acid sequence of SEQ ID NO:
• 20 and having 80% or more of the enzymatic activity of the amino acid sequence of SEQ ID NO:
• 20 at 50° C. pH 8,
• a protein consisting of an amino acid sequence including substitution, deletion, insertion, and/or addition of a total of one to thirty amino acid residues in the amino acid sequence consisting of amino acid residues 23-616 of the amino acid sequence of SEQ ID NO:
• 14 and having 80% or more of the enzymatic activity of amino acid residues 23-616 of the amino acid sequence of SEQ ID NO:
• 14 at 50° C. pH 8, and
• a protein consisting of an amino acid sequence including substitution, deletion, insertion, and/or addition of a total of one to thirty amino acid residues in the amino acid sequence consisting of amino acid residues 21-619 of the amino acid sequence of SEQ ID NO:
• 20 and having 80% or more of the enzymatic activity of amino acid residues 21-619 of the amino acid sequence of SEQ ID NO:
• 20 at 50° C. pH 8.

The mutant Escherichia coli according to claim 16, wherein said Escherichia coli is transformed with a recombinant DNA comprising a polynucleotide encoding a protein selected from the group consisting of

• a protein consisting of an amino acid sequence including substitution, deletion, insertion, and/or addition of a total of one to thirty amino acid residues in the amino acid sequence of SEQ ID NO:
• 14 and having 90% or more of the enzymatic activity of the amino acid sequence of SEQ ID NO:
• 14 at 50° C. pH 8,
• a protein consisting of an amino acid sequence including substitution, deletion, insertion, and/or addition of a total of one to thirty amino acid residues in the amino acid sequence of SEQ ID NO:
• 20 and having 90% or more of the enzymatic activity of the amino acid sequence of SEQ ID NO:
• 20 at 50° C. pH 8,
• a protein consisting of an amino acid sequence including substitution, deletion, insertion, and/or addition of a total of one to thirty amino acid residues in the amino acid sequence consisting of amino acid residues 23-616 of the amino acid sequence of SEQ ID NO:
• 14 and having 90% or more of the enzymatic activity of amino acid residues 23-616 of the amino acid sequence of SEQ ID NO:
• 14 at 50° C. pH 8, and
• a protein consisting of an amino acid sequence including substitution, deletion, insertion, and/or addition of a total of one to thirty amino acid residues in the amino acid sequence consisting of amino acid residues 21-619 of the amino acid sequence of SEQ ID NO:
• 20 and having 90% or more of the enzymatic activity of amino acid residues 21-619 of the amino acid sequence of SEQ ID NO:
• 20 at 50° C. pH 8.