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US 2012/0058510 A1Published application
Enzyme complex from trichoderma reesei and p. funiculosum enzymes
AssigneeDanisco A/S
InventorsNeville Marshall Fish, Lone Brønd Miller
Life of this patent
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Abstract
The invention relates to an improved enzyme complex having a plurality of enzyme activities of an expression product obtained by fermentation of the genus Trichoderma in combination with one or more enzymes of a different fungus strain.
Claims37
An enzyme complex derived from a combination of:
- a. An expression product obtained by fermentation of a species of the genus Trichoderma;
- and
- b. One or more enzymes of any one different species of the kingdom fungi selected from a xylanase (EC 3.2.1.8), a cellulase (EC 3.2.1.4), and a beta-glucanase (EC 3.2.1.6);
- and wherein at least about 61% of the beta-1,4-endoglucan hydrolase activity as measured by the “Assay 1” method as described herein is derived from fermentation of the genus Trichoderma.
1 more element
An enzyme complex derived from a combination of:
- a. At least about 61% of an expression product obtained by fermentation of the genus Trichoderma;
- and
- b. Less than about 39% of an expression product obtained by fermentation of a different fungus of the genus Penicillium;
- wherein the percentages are based on the beta-1,4-endoglucan hydrolase activity as measured by the “Assay 1” method as described herein.
1 more element
A process for the production of an enzyme complex, the process comprising the steps of
- a. fermentation of the genus Trichoderma in a medium to obtain a fermentation broth;
- b. fermentation of the genus Penicillium in a medium to obtain a fermentation broth, and
- c. recovery and combination of each enzyme complex derived from step a) and b) in the form of a cell free broth from said fermentations to obtain an enzyme complex, wherein at least about 61% of the beta-1,4-endoglucan hydrolase activity as measured by the “Assay 1” method as described herein is derived from fermentation of the genus Trichoderma.
Use of an enzyme complex in the production of fruit juice, wine, grain processing, fuel alcohol, and potable alcohol.
Dependencies are inferred from the claim text.