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US 2012/0058507 A1Published application
Clonal Derivation and Cell Culture quality Control Screening Methods
AssigneeSRU Biosystems, Inc.
InventorsSteven Shamah, Alexander Yuzhakov, Richard Wagner, Marla Abodeely, Bennett Rockney, Stephen C. Schulz, Zinkal Padalia, Michael Getman
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Abstract
The invention provides methods of label-free detection of changes in cell populations and mixed cell populations.
Claims14
A method for selecting one or more recombinant cells for expansion into clonal cell lines comprising:
- (a) transfecting a cell population with one or more transgenes to create a recombinant cell population;
- (b) seeding the recombinant cell population at a rate of one cell per well into a colorimetric resonant reflectance biosensor well plate;
- (c) allowing the recombinant cells to grow in the wells of the colorimetric resonant reflectance biosensor well plate for less than ten days;
- (d) optionally adding one or more test reagents to the wells;
- (e) assaying the recombinant cells in the wells for one or more functional attributes by detecting changes in peak wavelength values from the colorimetric resonant reflectance biosensor well;
- (f) selecting recombinant cells having the desired one or more functional attributes;
- and
- (g) expanding the recombinant cells having the desired one or more functional attributes into a clonal cell line.
5 more elements
A method for analyzing batch to batch variability of primary or stem cell cultures comprising:
- (a) selecting a standard primary cell culture or standard stem cell culture;
- (b) growing the standard primary cell culture or standard stem cell culture in one or more wells of a colorimetric resonant reflectance biosensor well plate and optionally adding one or more test reagents to the wells;
- (c) determining two or more functional attributes of the cells by detecting changes in peak wavelength value in the one or more wells and recording the two or more functional attributes of the standard primary cell culture or standard stem cell culture;
- (d) growing a test primary cell culture or a test stem cell culture in one or more wells of a colorimetric resonant reflectance biosensor well plate;
- optionally adding the one or more test reagents of step (b) to the cells;
- and assaying the test primary cell culture or the test stem cell culture by determining the same two or more functional attributes of step (c) by detecting changes in peak wavelength value in the one or more wells;
- (e) comparing the two or more functional attributes of standard primary cell culture or standard stem cell culture to the two or more functional attributes of test primary cell culture or test stem cell culture;
- wherein, variability of greater than 10% between each of the two or more of the functional attributes indicates that the test primary cell culture or test stem cell culture vary from the standard primary cell culture or standard stem cell culture.
5 more elements
A method for detecting expression of a transgene in transiently transfected cells comprising:
- (a) transiently transfecting a cell population with one or more transgenes to create a transiently transfected cell population;
- (b) placing the transiently transfected cells into the wells of the colorimetric resonant reflectance biosensor well plate;
- (c) optionally adding one or more test reagents to the wells;
- (d) assaying the transiently transfected cells in the wells for one or more functional attributes by detecting changes in peak wavelength values from the colorimetric resonant reflectance biosensor well;
- and
- selecting transiently transfected cells having the desired one or more functional attributes, wherein expression of a transgene in transiently transfected cells is detected.
3 more elements
Dependencies are inferred from the claim text.