Compositions and Methods for Characterizing Arthritic Conditions
OwnerAnthony Marottainventor
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Abstract
The present invention relates to autoantibodies against 14-3-3 proteins or circulating immune complexes thereof and the detection of such for the diagnosis and prognosis of an arthritic condition.
Claims26
A method for evaluating an arthritic condition in a subject, comprising:
- a) providing a sample from a subject suspected of having an arthritic condition;
- b) detecting the presence of an autoantibody against, or an immune complex with, at least one 14-3-3 protein or fragment thereof in said sample;
- wherein the presence of said autoantibody or immune complex is indicative of an arthritic condition in the subject.
The method according to any one of the preceding claims, wherein the biological sample is selected from the group consisting of blood, synovial fluid, plasma, serum or tissue.
The method according to any one of the preceding claims, wherein the 14-3-3 protein or fragment thereof is detectably labeled with a label selected from the group consisting of a radioactive label, a luminescent label, and a fluorescent label, and an enzyme.
The method according to any one of the preceding claims, wherein the 14-3-3 protein or fragment thereof is bound to a solid support.
The method according to any one of the preceding claims, wherein the autoantibody is detected by an ELISA assay.
The method according to any one of the preceding claims, wherein said detection occurs by chemiluminesence.
A kit for detecting the presence of an autoantibody to at least one 14-3-3 protein or fragment thereof in a sample, comprising at least one 14-3-3 protein or fragment thereof and a secondary reagent.
A method for determining the subtype of arthritis in a patient comprising (a) providing a first sample from a subject suspected of having an arthritic condition;
- (b) detecting the presence of an autoantibody against, or an immune complex with, at least one 14-3-3-protein or fragment thereof in said sample;
- (c) measuring the amount of said autoantibody or immune complex and comparing it against the amount of autoantibodies against, or immune complexes with, 14-3-3 in a second biological sample from a second subject whose subtype of arthritis is known and/or previously-established, wherein such comparison determines the subtype of arthritis for the first subject.
Dependencies are inferred from the claim text.