Recombinant fragments and synthetic peptides of 17-kDa polypeptide useful in detecting Bartonella henselae
AssigneeMedical Diagnostic Laboratories, LLC
InventorsLisa P. Huang, John G. Hoey, Martin E. Adelson, Eli Mordechai
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Abstract
The present disclosure describes recombinant and synthetic polypeptides of Bartonella henselae and related methods and kits for the detection of antibodies specific for Bartonella henselae. The 17-kDa polypeptides, methods and kits are useful in the detection of recent and/or ongoing infections with Bartonella henselae, which can be useful in the diagnosis of Cat Scratch Disease (CSD).
Claims32
A synthetic polypeptide having an amino acid sequence as set forth in SEQ ID NO:
- 22, whereby said synthetic polypeptide, when assayed in an ELISA assay, reacts to IFA sero-positive sera and does not react to IFA sero-negative sera from a patient infected with Bartonella henselae.
A recombinant polypeptide having an amino acid sequence as set forth in SEQ ID NO:
- 10, whereby said recombinant polypeptide, when assayed in an ELISA assay, reacts to IFA sero-positive sera and does not react to IFA sero-negative sera from a patient infected with Bartonella henselae.
A method for detection of Bartonella henselae in a biological sample from a mammal, comprising the steps of:
- (a) immobilizing a synthetic polypeptide to a solid support, wherein said synthetic polypeptide has an amino acid sequence selected from the group consisting of SEQ ID NO:
- 24, SEQ ID NO:
- 25, SEQ ID NO:
- 26, SEQ ID NO:
- 27, SEQ ID NO:
- 30, SEQ ID NO:
- 31, and SEQ ID NO:
- 32;
- (b) adding a biological sample onto said support to permit antibodies present in said biological sample to bind to said immobilized synthetic polypeptide;
- (c) washing to remove any unbound antibodies;
- and
- (d) detecting bound antibodies,
- wherein the presence of said bound antibodies is indicative of infection of said mammal with Bartonella henselae.
A method for detection of Bartonella henselae in a biological sample from a mammal, comprising the steps of:
- (a) immobilizing a recombinant polypeptide to a solid support, wherein said recombinant polypeptide has an amino acid sequence selected from the group consisting of SEQ ID NO:
- 10, SEQ ID NO:
- 42, SEQ ID NO:
- 43, SEQ ID NO:
- 44, and SEQ ID NO:
- 45;
- (b) adding a biological sample onto said support to permit antibodies present in said biological sample to bind to said immobilized recombinant polypeptide;
- (c) washing to remove any unbound antibodies;
- and detecting bound antibodies,
- wherein the presence of said bound antibodies is indicative of infection of said mammal with Bartonella henselae.
A vector comprising an isolated polynucleotide having a nucleotide sequence as set forth in SEQ ID NO:
- 9.
A method of preparing a recombinant polypeptide having an amino acid sequence as set forth in SEQ ID No:
- 10, comprising the steps of:
- (a) introducing an isolated polynucleotide into a host cell, said isolated polynucleotide having a nucleotide sequence as set forth in SEQ ID NO:
- 9;
- and
- (b) growing said host cell in a culture under suitable conditions to permit production of said recombinant polypeptide;
- and
- (c) isolating said recombinant polypeptide.
Dependencies are inferred from the claim text.