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AssigneeCarnegie Mellon University
InventorsMarcel P. Bruchez, Lauren A. Ernst, James Fitzpatrick, Chris Szent-Gyorgyi, Brigitte F. Schmidt, Alan Waggoner
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The present invention presents designs for high extinction quenched “dyedrons” that can be activated by conversion of a single acceptor/quencher in the molecular assembly to a fluorescent state. The quencher is activated by noncovalent binding to a unique complementary expressible fluorogen activating peptide (FAP). In this way, the quencher serves as the homogeneous switch, receiving energy efficiently from each of the donor molecules of the dendronic antenna, and releasing it as fluorescence only when activated by binding. The sum of the extinction of the multiple dyes on the antenna will provide dramatic enhancements in the effective brightness of the probe in standard imaging systems. This approach provides a set of probes with exceptional brightness, specifically targeted to an expressed tag that activates the fluorescence of the dyedron.
A compound comprising two or more excitable donor moieties, linked to a single activatable acceptor moiety, wherein the donor moieties transfer at least 50% of their excitation energy to the acceptor moiety, and when activated, the acceptor moiety produces a detectable fluorescent signal when the donor moieties are excited.
An activator polypeptide comprising a sequence chosen from:
Dependencies are inferred from the claim text.