Method for induction of differentiation of es cell
AssigneeKumamoto University
InventorsShoen Kume, Nobuaki Shiraki, Kahoko Umeda, Kazuhiko Kume
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Abstract
It is an object of the present invention to establish a system for reliably differentiating an ES cell into a hepatic cell. The present invention provides a method for inducing the differentiation of an ES cell into a hepatic cell, which comprises, in the presence of an M15 cell, culturing a mammal-derived ES cell in the presence of activin and bFGF, and then culturing the ES cell in the presence of dexamethasone, HGF, and oncostatin M.
Claims12
A method for inducing the differentiation of an ES cell into a hepatic cell, which comprises, in the presence of an M15 cell, culturing a mammal-derived ES cell in the presence of activin and bFGF, and then culturing the ES cell in the presence of dexamethasone, HGF, and oncostatin M.
A method for screening for a substance capable of promoting or inhibiting the induction of the differentiation of an ES cell into a hepatic cell, wherein, when a mammal-derived ES cell is induced to differentiate into a hepatic cell by culturing, in the presence of an M15 cell, the ES cell in the presence of activin and bFGF and then culturing the ES cell in the presence of dexamethasone, HGF, and oncostatin M, the ES cell is cultured in the presence of a test substance, and the level of induction of the differentiation into a hepatocyte in a case of culturing ES cells in the absence of the test substance is then compared with the level of induction of the differentiation into a hepatocyte in a case of culturing the ES cells in the presence of the test substance.
Dependencies are inferred from the claim text.