Method for detecting an antigen
AssigneePanasonic Corporation
InventorsKazuaki NISHIO, Nozomu Matsukawa, Shigeo Yoshii
Life of this patent
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Abstract
Provided is a method for detecting an antigen without use of a labeled-antibody. A support having an antibody and a multi-copper oxidase CueO immobilized thereon is brought into contact with a first buffer solution containing the antigen, a current is measured by a potentiostat method using the support and a second buffer solution, and when the measured current is greater than or equal to 1.5×(blank value), it is determined that the antigen exists. The second buffer solution contains a substrate of the CueO and has an ionic strength falling within a range of not less than 0.3 mM and not more than 1.0 mM.
Claims7
A method for detecting an antigen, the method comprising steps of:
- a step (A) of bringing a support into contact with a first buffer solution to be predicted to contain the antigen, wherein
- an antibody and a multi-copper oxidase CueO are immobilized on the support,
- the support is electrically conductive,
- the antibody reacts specifically to the antigen,
- a step (B) of measuring a current by a potentiostat method using the support and a second buffer solution,
- wherein the second buffer solution contains a substrate of the multi-copper oxidase CueO,
- the second buffer solution has an ionic strength falling within a range of not less than 0.3 mM and not more than 1.0 mM, and
- a step (C) of determining that the antigen exists in the first buffer solution if the following inequality is satisfied:
- the current measured in the step (B)>=1.5×(blank value)
- wherein the blank value is a current measured with a potentiostat method in which the antigen is not used but the second buffer solution is used.
Dependencies are inferred from the claim text.