US 2012/0058487 A1 · COMPOSITIONS AND METHODS FOR DETECTING KLEBSIELLA PNEUMONIAE

Abstract

Four highly conserved genes, encoding translation elongation factor Tu, translation elongation factor G, the catalytic subunit of proton-translocating ATPase and the RecA recombinase, are used to generate species-specific, genus-specific, family-specific, group-specific and universal nucleic acid probes and amplification primers to rapidly detect and identify algal, archaeal, bacterial, fungal and parasitical pathogens from clinical specimens for diagnosis. The detection of associated antimicrobial agents resistance and toxin genes are also under the scope of the present invention.

Claims19

Dependent claims

A composition for the detection of Klebsiella pneumoniae in a sample using a nucleic acid amplification assay, comprising an amplification primer pair, said amplification primer pair consisting of an oligonucleotide consisting of SEQ ID NO:

1331 or the complement thereof, and an oligonucleotide consisting of SEQ ID NO:1332 or the complement thereof, or variants of either SEQ ID NO:
1331 or 1332, or both,
wherein said variants optionally have up to three nucleotide changes compared to SEQ ID NO:1331 or 1332,
wherein said variants are capable of hybridizing to and amplifying K. pneumoniae nucleic acids in said nucleic acid amplification assay,
and
wherein each oligonucleotide optionally includes a detectable moiety.

3 more elements

Dependencies are inferred from the claim text.

Facts

Application

11/842,141Filed 21 Aug 2007

Priority

CA CA 2,283,45828 Sep 1999

Published

8 Mar 2012

Term ends

Not estimatedThe source records don’t support an estimate.

Classification14 codes

C12Q 1/6895
For detection or identification of organisms: for plants, fungi or algae
A61P 31/00
Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
A61P 31/04
Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics: antibacterial agents
A61P 31/10
Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics: antimycotics

Family

Application10/089,177Continuation · filed 27 Mar 2002

Application11/236,785Continuation · filed 27 Sep 2005

Application11/842,141Continuation

US 2009/0068641 A1Published application

US 8,114,601 B2Related patent

US 8,182,996 B2Related patent

US 2012/0058487 A1This patent

From the US filing history. Foreign family members aren’t listed.

Compositions and methods for detecting klebsiella pneumoniae

Life of this patent

Compositions and methods for detecting klebsiella pneumoniae

Life of this patent

Abstract

Claims19