Mitochondrial mutations and rearrangements as a diagnostic tool for the detection of sun exposure, prostate cancer and other cancers
AssigneeMitomics Inc.
InventorsRyan Parr, Robert Thayer, Gabriel Dakubo, Jennifer Maki, Kerry Robinson, Andrea Maggrah, Brian Reguly, Andrew Harbottle, Mark Birch-Machin
Life of this patent
Estimating the term…
Abstract
Mitochondrial DNA deletions useful for the detection of cancers and sun exposure are provided. In particular, methods and kits for detecting mitochondrial DNA deletions for the early detection, diagnosis and progression of prostate cancer, sun exposure and non-melonoma skin cancer are provided.
Claims41
A method of detecting a deletion spanning approximately nucleotides 547 to 4443 in the minor arc of the human mtDNA genome, wherein said deletion is associated with skin cancer and/or UV exposure, comprising:
- (a) providing a biological sample;
- (b) extracting mtDNA from the biological sample;
- and
- (c) detecting the presence of the deletion in the mtDNA.
A method for determining cumulative UV exposure or for monitoring long term safety of clinical UV phototherapy regimes or for monitoring a person for sun exposure and associated non-melanoma skin cancer (NMSC), in a subject having mtDNA, the method comprising:
- (a) providing a biological sample from the subject;
- (b) extracting mtDNA from the biological sample;
- and
- (c) detecting the presence of a deletion spanning approximately nucleotides 547 to 4443 in the minor arc of the human mtDNA genome.
Use of an mtDNA deletion spanning approximately nucleotides 547 to 4443 in the minor arc of the human mtDNA genome as a biomarker for UV damage or as a biomarker for sking cancer.
An array for use in detecting skin cancer, UV exposure, UV damage and/or monitoring associated non-melanoma skin cancer (NMSC), the array comprising at least one nucleic acid member, and a solid substrate, wherein the at least one nucleic acid member is associated with a human mtDNA deletion spanning approximately nucleotides 547 to 4443 of the human mtDNA genome, and wherein the at least one nucleic acid member has a unique position on said array and is stably associated with the solid substrate.
A kit for detecting skin cancer, UV exposure, UV damage and/or monitoring associated non-melanoma skin cancer (NMSC), the kit comprising a means for detecting a mtDNA deletion spanning approximately nucleotides 547 to 4443 in the minor arc of the human mtDNA genome, and at least one member selected from:
- a solid support, means for holding the solid support, a disposable chip, means for holding the disposable chip, a means for extraction of mitochondrial DNA, primers, reagents and instructions.
An array for use in detecting prostate cancer, the array comprising at least one nucleic acid member, and a solid substrate, wherein the at least one nucleic acid member is associated with a human mtDNA deletion spanning approximately nucleotides 10744 to 14124 of the human mtDNA genome, and wherein the at least one nucleic acid member has a unique position on said array and is stably associated with the solid substrate.
A kit for detecting prostate cancer, the kit comprising a means for detecting a mtDNA deletion spanning approximately nucleotides 10744 to 14124 of the human mtDNA genome, and at least one member selected from:
- a solid support, means for holding the solid support, a disposable chip, means for holding the disposable chip, a means for extraction of mitochondrial DNA, primers, reagents and instructions.
Dependencies are inferred from the claim text.