Antigenic approach to the detection and isolation of microparticles associated with fetal dna
OwnerDorothy E. Lewis, Aaron F. Orozco +1inventors
InventorsDorothy E. Lewis, Aaron F. Orozco, Farideh Z. Bischoff
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Abstract
The present disclosure provides methods of quantifying and isolating microparticles of fetal from maternal bodily fluids such as cell free maternal plasma. Antibodies or combinations of antibodies that selectively bind fetal microparticles in maternal bodily fluid permit quantification by flow cytometry and isolation through flow cytometry based sorting and immunopurification.
Claims19
A method of enriching microparticles of fetal origin having fetal nucleic acids from a maternal sample, the method comprising the steps of
- a) combining in a volume of liquid
- i) an antibody that binds to a fetal specific antigen and
- ii) a microparticle of fetal origin, wherein the microparticle includes the fetal specific antigen,
- b) forming an immunocomplex comprising the microparticles and the antibody, and
- c) isolating the immunocomplex from the volume of liquid.
A method of enriching microparticles of fetal origin having fetal nucleic acids from a maternal sample, the method comprising the steps of
- a) combining in a volume of liquid
- i) an antibody that binds to a fetal specific antigen and
- ii) a microparticle of fetal origin, wherein the microparticle includes the fetal specific antigen,
- b) forming an immunocomplex comprising the microparticles and the antibody, and
- c) enriching the immunocomplex.
A method of detecting a fetal nucleic acid, the method comprising the steps of:
- a) enriching a microparticle of fetal origin having a fetal nucleic acid from a maternal sample by combining in a volume of liquid an antibody that binds to a fetal specific antigen and a microparticle of fetal origin, wherein the microparticle includes the fetal specific antigen, wherein the microparticle and the antibody form an immunocomplex;
- and
- b) performing a diagnostic test on the microparticle.
The method of any one of the preceding claims further comprising one or more further purifications of the microparticles.
Dependencies are inferred from the claim text.