Multimers of s. solfataricus single-stranded dna-binding protein and methods of use thereof
AssigneeThe Regents of the University of California
InventorsCynthia A. Haseltine, Stephen C. Kowalczykowski
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Abstract
The invention provides multimers of S. solfataricus ssDNA binding protein that bind single stranded DNA. The multimers are robust and stable reagents for use in PCR and other techniques for engineering DNA. The invention further provides methods for performing nucleic acid amplification and engineering using the multimers.
Claims16
An isolated multimer, wherein each unit of said multimer has at least 70% sequence identity to SEQ ID NO:1, and wherein said multimer binds single stranded DNA.
A method of performing nucleic acid amplification, said method comprising contacting a single stranded DNA with a multimeric protein, wherein each unit of said multimeric protein has at least 70% sequence identity to SEQ ID NO:1, and wherein said multimeric protein binds single stranded DNA.
A method for performing nucleic acid engineering, comprising contacting single stranded DNA with a multimeric protein, wherein each unit of said multimeric protein has at least 70% sequence identity to SEQ ID NO:1, and wherein said multimeric protein binds single stranded DNA.
Dependencies are inferred from the claim text.