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US 2012/0058477 A1Published application
Method for detecting the expression of cyclin b2 gene by real-time quantitative pcr
OwnerXiaoming Donginventor
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Abstract
A method and a kit for quantitative detection of the expression of cyclin B2 gene in blood samples, especially in serum.
Claims10
An in vitro method of detecting the expression of cyclin B2 gene in a sample, comprising:
- preparing a DNA standard based on nucleotides 1078-1316 segment of cyclin B2 gene;
- designing primers and Taqman probes based on nucleotides 1144-1243 segment of cyclin B2 gene;
- and
- using Taqman technology to relatively quantitative analyse the mRNA of cyclin B2 gene in the sample.
1 more element
A kit for quantitatively detecting the expression of cyclin B2 gene in a serum sample, characterized in that it comprises primers, DNA standard and Taqman probes, wherein said primers and probes are designed based on nucleotides 1144-1243 segment of cyclin B2 gene, said DNA standard is prepared based on neucleotides 1078-1316 segment of cyclin B2 gene.
Dependencies are inferred from the claim text.