Differential immunoassay for prrs vaccine antibody
AssigneeIowa State University Research Foundation, Inc.
InventorsKyoung-Jin Yoon, Wai-Hong Wu
Life of this patent
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Abstract
The present invention relates to immunoassays for serologically differentiating animals naturally infected with PRRS virus from animals vaccinated against PRRS. The immunoassays provide detection of at least a portion of the N terminal region of the 2b portion of PRRSV. The immunoassay is preferably an enzyme-linked immunosorbent assay (ELISA).
Claims9
A method of determining whether a pig has received a commercial PRRSV Modified live attenduated vaccine (MLV) comprising:
- obtaining a biological sample from an individual;
- contacting the biological sample with an antibody that binds to the N terminal region of the 2b protein of PRRSV to form a complex when there is an aspartic acid at position 9;
- and
- assaying for the presence of said complex, wherein an aspartic acid at position 9 indicates that said pig has not received a commercial PRRSV Modified live attenduated vaccine (MLV).
A method of determining whether a pig has received a commercial PRRSV Modified live attenduated vaccine (MLV) a biological sample comprising:
- determining the amino acid present at position 9 of the 2b protein of PRRSV in said sample, wherein an aspartic acid at position 9 indicates that a pig has not received a commercial PRRSV Modified live attenduated vaccine (MLV) and a tyrosine or glycine at said position indicates that said pig has received said commercial PRRSV Modified live attenduated vaccine (MLV).
Dependencies are inferred from the claim text.