Cold-active beta-galactosidase, a method of producing same and use of such enzyme
AssigneeKobenhavns Universitet
InventorsPeter Stougaard, Mariane Schmidt
Life of this patent
Estimating the term…
Abstract
A novel cold-active beta-galactosidase is enzyme specific for lactose. The enzyme is thus useful in e.g. the food industry for catalyzing at low temperatures the hydrolysis of lactose disaccharide into its constituent monosaccharides, glucose and galactose. A method produces the cold-active beta-galactosidase by recombinant DNA technology.
Claims24
A purified cold-active beta-galactosidase having the amino acid sequence as defined in SEQ ID NO. 1 or one having at least 80% homology to the amino acid sequence as defined in SEQ ID NO. 1, the amino acid sequence being selected so that the enzyme has a stable enzymatic activity at temperatures less than 8° C.
An isolated DNA sequence, which a) encodes a protein with an amino acid sequence as given in SEQ ID NO. 2, or b) hybridises under stringent conditions to the sequence of a), or c) is degenerative of the sequence of a) or b)
(canceled)
Dependencies are inferred from the claim text.