Centrifugal device and method for performing binding assays

A device for measuring the binding of a first partner in an interaction to a second partner in said interaction, wherein said interaction partners are molecular entities, said device

• comprising:
• a) an opaque temperature-controlled chamber having a centrifuge rotor therein, said rotor having at or near the periphery of the rotor and attached thereto at least one radially positioned transparent reaction well, said reaction well having on an upper surface thereof an aperture for the addition of reagents to the reaction well, said reaction well further including on an internal surface thereof at the end closest the axis of said rotor at least one attachment zone for said second interaction partner,
• b) a stationary system for detecting light emitted or absorbed by said first interaction partner or an indicator molecule bound thereto;
• and
• c) means for controlling the temperature of said chamber and the operation of said rotor.