System and method for fluorescence guided ingredient specific particle sizing
AssigneeChemImage Corporation
InventorsRyan Priore, Oksana Olkhovyk, Michael Fuhrman
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Abstract
The present disclosure provides for a system and method for rapid, accurate, and reliable targeting and interrogation of pharmaceutical samples. An autofluorescence image of a sample may be generated and analyzed to identify areas of interest that exhibit autofluorescence characteristic of APIs. These areas of interest may then be targeted for analysis using Raman chemical imaging. This Raman chemical image may be used to determine geometric properties of particles present in a sample such as size and particle distribution.
Claims22
A method comprising:
- generating at least one autofluorescence image representative of a sample, wherein said sample comprises at least one particle associated with an active ingredient of interest;
- analyzing said autofluorescence image to thereby identify a plurality of regions of interest, wherein each said region of interest exhibits autofluorescence characteristic of at least one active ingredient of interest;
- targeting each said region of interest to thereby generate at least one Raman chemical image representative of each region of interest;
- and
- analyzing said Raman chemical image to thereby determine at least one geometric property of said particle.
A system comprising:
- a first illumination source configured so as to illuminate at least a portion of a sample to thereby generate a first plurality of interacted photons, wherein said sample comprises at least one particle associated with an active ingredient of interest;
- a first detector configured so as to detect said first plurality of interacted photons and generate at least one autofluorescence image representative of said sample;
- a means for analyzing said autofluorescence image to thereby identify at least one region of interest of said sample, wherein each said region of interest exhibits autofluorescence characteristic of at least one active ingredient of interest;
- a second illumination source configured to illuminate at least one said region of interest to thereby generate a second plurality of interacted photons;
- a filter configured so as to sequentially filter said second plurality of interacted photons into a plurality of predetermined wavelength bands;
- a second detector configured so as to detect said second plurality of interacted photons and generate at least one Raman chemical image representative of said region of interest;
- and
- a means for analyzing said Raman chemical image to thereby determine at least one geometric property representative of said particle.
A storage medium containing machine readable program code, which, when executed by a processor, causes said processor to perform the following:
- generate at least one autofluorescence image representative of a sample, wherein said sample comprises at least one particle associated with an active ingredient of interest;
- analyze said autofluorescence image to thereby identify a plurality of regions of interest, wherein each said region of interest exhibits autofluorescence characteristic of at least one active ingredient of interest;
- target each said region of interest to thereby generate at least one Raman chemical image representative of each region of interest;
- and
- analyze said Raman chemical image to thereby determine at least one geometric property of said particle.
Dependencies are inferred from the claim text.