Fluorescence intensity compensation method and fluorescence intensity computing apparatus
AssigneeSony Corporation
InventorYoshitsugu Sakai
Life of this patent
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Abstract
A fluorescence intensity compensation method includes emitting light onto microparticles multiply labeled with a plurality of fluorochromes having fluorescence wavelength ranges that overlap each other in order to excite the fluorochromes and receiving fluorescence generated by the excited fluorochromes using photodetectors having different reception wavelength ranges, and computing fluorescence intensities of the fluorochromes by compensating detection values of the photodetectors under a predetermined constraint condition imposed on the computed fluorescence intensities.
Claims7
A fluorescence intensity compensation method comprising:
- emitting light onto a microparticle multiply labeled with a plurality of fluorochromes having fluorescence wavelength ranges that overlap each other in order to excite the fluorochromes and receiving fluorescence generated by the excited fluorochromes using photodetectors having different reception wavelength ranges;
- and
- computing fluorescence intensities of the fluorochromes by compensating detection values of the photodetectors under a predetermined constraint condition imposed on the computed fluorescence intensities.
A fluorescence intensity computing apparatus comprising:
- a measurement unit configured to emit light onto microparticles multiply labeled with a plurality of fluorochromes having fluorescence wavelength ranges that overlap each other in order to excite the fluorochromes and receive fluorescence generated by the excited fluorochromes using photodetectors having different reception wavelength ranges;
- and
- a computing unit configured to compute fluorescence intensities of the fluorochromes by compensating detection values of the photodetectors under a predetermined constraint condition imposed on the computed fluorescence intensities.
Dependencies are inferred from the claim text.