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US 2012/0040399 A1Published application
Method for the production of recombinant polyomaviral vector particles
AssigneeAmarna Holding B.V.
InventorWalter Gerhardus De Vries
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AssigneeAmarna Holding B.V.
InventorWalter Gerhardus De Vries
Estimating the term…
The present invention relates to improved methods for the production of viral particles, viral vector particles and recombinant proteins. In particular, the invention relates to improved methods for the production of recombinant polyomaviral vector particles and polyomaviral vector production cell lines. More in particular, the invention relates to methods for the production of simian polyomaviral vector particles such as simian virus 40 (SV40) viral vector particles. The invention also relates to compositions comprising viral vectors and uses thereof and viral vector particles to treat genetic disorders, transplant rejection, autoimmune diseases, infectious diseases, allergies or cancer. The invention also relates to methods for the production of recombinant proteins in mammalian cells and methods to enhance the production of recombinant proteins in mammalian cells.
Method for the production of recombinant polyomaviral vector particles not encoding a functional polyomaviral small T antigen, the method comprising:
A composition comprising more than one million recombinant polyomaviral vector particles not encoding a functional polyomaviral small T antigen, said polyomavirus particles being incapable of replicating in cells that are permissive for the wildtype polyomavirus and do not express a functional polyomaviral large T antigen, wherein the composition does not contain a single polyomavirus particle being able to replicate in cells which are permissive for the wildtype polyomavirus wherein said cells do not express a functional polyomaviral large T antigen.
A cell line permissive for a polyomavirus, said cell line encoding a functional polyomaviral large T antigen and not encoding a functional polyomaviral small T antigen.
(canceled)
Dependencies are inferred from the claim text.