Methods for differentiating plasma- derived protein from recombinant protein in a sample
AssigneesBaxter Healthcare S.A., Baxter International Inc.
InventorsAlfred Weber, Peter Turecek, Hans-Peter Schwarz
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Abstract
The present invention relates, in general, to methods for detecting and quantitating plasma-derived protein and recombinant protein in a sample based on the difference in protein glycosylation, when the plasma protein and the recombinant protein are essentially the same protein.
Claims12
1-18. (canceled)
A method to differentiate plasma-derived protein and recombinant protein in a sample comprising, contacting the sample with a composition comprising a lectin protein;
- detecting binding of the lectin to the protein;
- and
- comparing the amount of binding of protein-bound lectin in the sample to a lectin:protein binding curve to determine the amount of plasma-derived protein in the sample.
The method of claim 18 wherein the lectin is Sambucus nigra agglutinin (SNA).
A kit for quantitating levels of plasma derived protein and recombinant protein in a sample, wherein the plasma-derived protein and the recombinant protein encode the same protein, the kit comprising
- a protein binding agent;
- a composition comprising a lectin specific for a carbohydrate on the plasma-derived protein and a detectable label;
- and
- a protein standard.
Dependencies are inferred from the claim text.