Pre-coated surfaces for analysis

A method of producing a pre-coated substrate comprising:

• (a) treating a substrate to generate a surface comprising a first part that is more hydrophilic than a second part;
• and
• (b) applying and evaporating a matrix compound solution onto the surface, wherein the matrix compound is deposited primarily onto the more hydrophilic part to produce the pre-coated substrate.

36-45. (canceled)

The method of claim 44, wherein the microcontact printing further comprises:

• (i) depositing a first compound onto the substrate to generate the more hydrophilic first part;
• and
• (ii) depositing a more hydrophobic compound onto the parts of the substrate where the first compound is not located to generate the second part.

The method of claim 44, wherein the microcontact printing further comprises:

• (i) depositing a first compound onto the substrate to generate the second part;
• and
• (ii) depositing a more hydrophilic compound onto the parts of the substrate where the first compound is not located to generate the more hydrophilic first part.

48-60. (canceled)

(canceled)

(canceled)

A method of producing a pre-coated substrate comprising:

• (a) treating a substrate to generate a surface comprising a first part that is more hydrophilic than a second part;
• and
• (b) applying and evaporating the matrix compound solution onto the surface, wherein the matrix compound is deposited primarily onto the more hydrophobic part to produce the pre-coated substrate.

(canceled)

(canceled)

A method of producing a pre-coated substrate comprising:

• (a) treating a substrate to generate a surface comprising a first part that is more hydrophilic than a second part;
• and
• (b) immersing the surface into and removing the surface from a target compound solution, wherein the target compound is deposited primarily onto the more hydrophilic part to produce the pre-coated substrate.

71-80. (canceled)

The method of claim 79, wherein the microcontact printing further comprises:

• (i) depositing a first compound onto the substrate to generate the more hydrophilic first part;
• and
• (ii) depositing a more hydrophobic compound onto the parts of the substrate where the first compound is not located to generate the second part.

The method of claim 79, wherein the microcontact printing further comprises:

• (i) depositing a first compound onto the substrate to generate the second part;
• and
• (ii) depositing a more hydrophilic compound onto the parts of the substrate where the first compound is not located to generate the more hydrophilic first part.

83-98. (canceled)

101-103. (canceled)

A pre-coated substrate comprising a surface comprising a first part that is more hydrophilic than a second part, wherein the first part contains a target compound.

105-110. (canceled)

111-129. (canceled)