Influenza hemagglutinin and neuraminidase variants
AssigneeMedImmune, LLC
InventorsChin-Fen Yang, George Kemble
Life of this patent
Estimating the term…
Abstract
Polypeptides, polynucleotides, methods, compositions, and vaccines comprising (avian pandemic) influenza hemagglutinin and neuraminidase variants are provided.
Claims20
A 6:2 reassortant influenza virus, wherein said virus comprises 6 internal genome segments from one or more donor viruses other than A/Ann Arbor/6/60 and 2 surface antigen genome segments, wherein the surface antigen genome segments encode an HA and a NA polypeptide, wherein the surface antigen genome segment that encodes the HA polypeptide produces a polypeptide comprising the amino acid sequence of SEQ ID NO:41.
A method for producing an influenza virus in cell culture, the method comprising:
- i) introducing into a population of host cells, which population of host cells is capable of supporting replication of influenza virus, a plurality of vectors comprising nucleotide sequences corresponding to:
- (a) at least 6 internal genome segments of a first influenza strain, wherein the first influenza strain is not A/Ann Arbor/6/60;
- and, at least one genome segment encoding an HA surface antigen polypeptide wherein the surface antigen polypeptide comprises the amino acid sequence of SEQ ID NO:
- 41;
- or
- (b) at least 6 internal genome segments of a first influenza strain, wherein the first influenza strain is not A/Ann Arbor/6/60 and which influenza strain comprises one or more phenotypic attributes selected from the group consisting of:
- attenuated, cold adapted and temperature sensitive;
- and, at least one genome segment encoding an HA surface antigen wherein the surface antigen polypeptide comprises the amino acid sequence of SEQ ID NO:
- 41,
- ii) culturing the population of host cells at a temperature less than or equal to 35° C.;
- and,
- iii) recovering an influenza virus.
Dependencies are inferred from the claim text.