Cytochrome P450s and uses thereof
AssigneeUniversity of Kentucky Research Foundation
InventorsJoseph Chappell, Lyle F. Ralston
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Abstract
The invention features isolated cytochrome P450 polypeptides and nucleic acid molecules, as well as expression vectors and transgenic plants containing these molecules. In addition, the invention features uses of such molecules in methods of increasing the level of resistance against a disease caused by a plant pathogen in a transgenic plant, in methods for producing altered compounds, for example, hydroxylated compounds, and in methods of producing isoprenoid compounds.
Claims17
A method for producing an isoprenoid compound, comprising:
- a) providing a host cell that expresses heterologous nucleic acid encoding a first recombinant protein comprising an isoprenoid synthase, and a second recombinant protein comprising a cytochrome P450 polypeptide;
- b) culturing the host cell under conditions suitable for expressing the first recombinant protein comprising an isoprenoid synthase and the second recombinant protein comprising the cytochrome P450 polypeptide and under conditions for producing an isoprenoid compound, wherein the first and second recombinant protein together catalyze the formation of an isoprenoid compound not normally produced by said host cell;
- and
- c) recovering the isoprenoid compound.
A method for producing an altered isoprenoid compound, comprising:
- a) contacting an isoprenoid compound with an isolated cytochrome P450 polypeptide, wherein the P450 polypeptide catalyzes the formation of an isoprenoid compound, under conditions allowing for the dual hydroxylation, oxidation, demethylation, methylation, or any combination thereof of said compound to produce an altered isoprenoid compound;
- and
- b) recovering the altered isoprenoid compound.
A host cell, comprising nucleic acid encoding a first recombinant protein comprising an isoprenoid synthase, and nucleic acid encoding a second recombinant protein comprising a cytochrome P450 polypeptide, wherein:
- the P450 polypeptide catalyzes the formation of an isoprenoid compound under conditions allowing for dual hydroxylation, oxidation, demethylation, methylation, or any combination thereof of said compound;
- the first and second recombinant proteins together catalyze the formation of an isoprenoid compound not normally produced by said host cell;
- and
- the first recombinant protein and the nucleic acid encoding the second recombinant protein are heterologous to the host cell.
Dependencies are inferred from the claim text.