Microorganisms and methods for the production of ethylene glycol
OwnerRobin E. Osterhout, Priti Pharkya +1inventors
InventorsRobin E. Osterhout, Priti Pharkya, Anthony P. Burgard
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Abstract
The invention provides non-naturally occurring microbial organisms having an ethylene glycol pathway. The invention additionally provides methods of using such organisms to produce ethylene glycol.
Claims23
A non-naturally occurring microbial organism, comprising a microbial organism having an ethylene glycol pathway comprising at least one exogenous nucleic acid encoding an ethylene glycol pathway enzyme expressed in a sufficient amount to produce ethylene glycol, said ethylene glycol pathway comprising a serine decarboxylase, a serine aminotransferase, a serine oxidoreductase (deaminating), a hydroxypyruvate decarboxylase, a glycolaldehyde reductase, an ethanolamine aminotransferase, an ethanolamine oxidoreductase (deaminating), a hydroxypyruvate reductase or a glycerate decarboxylase.
A non-naturally occurring microbial organism, comprising a microbial organism having an ethylene glycol pathway comprising at least one exogenous nucleic acid encoding an ethylene glycol pathway enzyme expressed in a sufficient amount to produce ethylene glycol, said ethylene glycol pathway comprising a hydroxypyruvate decarboxylase, a glycolaldehyde reductase, a hydroxypyruvate reductase, a glycerate decarboxylase, a 3-phosphoglycerate phosphatase, a glycerate kinase, a 2-phosphoglycerate phosphatase, a glycerate-2-kinase or a glyceraldyhyde dehydrogenase.
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Dependencies are inferred from the claim text.