System for detection of spaced droplets

A detection system for droplet-based assays, comprising:

• a tip;
• a channel network defining a flow path for droplets, the flow path extending from the tip, through at least two confluence regions arranged in series, each confluence region configured to increase an average distance between droplets, and to an examination region disposed downstream of the confluence regions;
• and
• a detector operatively connected to the examination region.

A method of detection for droplet-based assays, comprising:

• driving droplets along a flow path from a tip, through a confluence region where a dilution fluid is introduced into the flow path, and through an examination region disposed downstream of the confluence region;
• and
• detecting light from the examination region as droplets pass through,
• wherein the flow path has a minimum diameter adjacent the confluence region.