Lantibiotic biosynthetic gene clusters from a. garbadinensis and a. liguriae
AssigneeNovacta Biosystems Limited
InventorsSteven Boakes, Jesus Cortes Bargallo, Michael John Dawson
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Abstract
This invention relates to characterisation of the biosynthetic gene cluster for the lantibiotic actagardine, identification of a novel variant of actagardine and its biosynthetic cluster, and methods of production and use of actagardine, a novel actagardine variant, herein referred to as actagardine B, and variants of both of these produced according to this invention, utilizing genes from the characterised biosynthetic gene clusters.
Claims23
1-43. (canceled)
A method for producing a host cell suitable for expressing a type B lantibiotic peptide deoxyactagardine, actagardine or a variant thereof, the method comprising the step of transforming a cell by introducing:
- a lanA gene, said gene encoding a LanA polypeptide comprising actagardine, actagardine B, ala(0)-actagardine, ala(0)-actagardine B or an amino acid variant of any one of the same wherein:
- 1 to 5 amino acids in the sequence is/are substituted by another amino acid, or
- the amino acid sequence is at least 90% identical thereto,
- optionally together with one or more associated cluster genes suitable for conversion of a precursor peptide into said peptide,
- thereby generating a host cell comprising a polynucleotide sequence encoding said peptide and suitable for expressing same.
A vector suitable for transforming a host cell in the method defined in claim 1 said vector comprising an expression construct comprising a gene encoding a LanA peptide comprising actagardine, actagardine B, ala(0)-actagardine, ala(0)-actagardine B or a variant of any one of same wherein:
- 1 to 5 amino acids in the peptide are substituted by another amino acid, or
- the amino acid sequence is at least 90% identical thereto,
- optionally together with an associated cluster genes required for conversion of a precursor peptide into a product peptide.
A host cell selected from the group comprising ΔLigA and ΔActA.
Dependencies are inferred from the claim text.