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AssigneeNovozymes Biopharma DK A/S
InventorsHendrik Van Urk, David John Mead, Philip Harvey Morton, Andrew John Cartwright, Jason Cameron, David James Ballance, Michel Gaston Joseph Grandgeorge, Stephen Berezenko, John Rodney Woodrow, Darrell Sleep, Jean-Luc Bernard Veron
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A process is provided for the preparation of a highly pure albumin solution the process comprising subjecting albumin (preferably expressed and secreted by transformed yeast) to a series of chromatographic steps. Preferably, the process comprises the steps of positive mode cation exchange chromatography, positive mode anion exchange chromatography, positive mode affinity chromatography, negative mode affinity chromatography (preferably using immobilised aminophenylboronic acid), negative mode cation exchange chromatography, and negative or positive mode anion exchange chromatography. A process for reducing the level of nickel in an albumin solution is also disclosed, as is a recombinant albumin coding sequence comprising two or more in-frame translation stop codons. Also disclosed is a process for producing recombinant albumin, the process comprising culturing a fungal cell expressing a is recombinant albumin coding sequence, wherein the cell has a reduced capacity of mannosylation of the recombinantly-expressed albumin.
1-53. (canceled)
A process for purifying an albumin solution, the process comprising the step of subjecting a first albumin solution of pH 8.0-9.5 buffered with a buffer comprising a divalent cation at a concentration of 5-250 mM, and having a conductivity in the range of 1 to 75 mS.cm−1, to an affinity chromatography step which is run in negative mode with respect to the albumin and which utilises an affinity matrix comprising immobilised dihydroxyboryl groups, thereby obtaining a purified albumin solution.
Dependencies are inferred from the claim text.