Composition and Methods Related to Modification of 5-Hydroxymethylcytosine (5-hmC)
AssigneeThe University of Chicago, Uchicago Tech
InventorsChuan He, Liang Zhang, Chunxiao Song
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Abstract
The present invention relates generally to the field of molecular biology. More particularly, it concerns methods and compositions for detecting, evaluating, and/or mapping 5-hydroxymethyl-modified cytosine bases within a nucleic acid molecule.
Claims34
A method for distinguishing 5-hydroxymethylcytosine from 5-methylcytosine in a nucleic acid molecule comprising incubating the nucleic acid molecule with β-glucosyltransferase and a modified uridine diphosphoglucose (UDP-Glu) molecule to glycosylate 5-hydroxymethylcytosines in the nucleic acid molecule with a modified glucose molecule.
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A method for mapping 5-hydroxymethylcytosine in a nucleic acid molecule comprising incubating the nucleic acid molecule with β-glucosyltransferase and a modified uridine diphosphoglucose (UDP-Glu) molecule to glycosylate 5-hydroxymethylcytosines in the nucleic acid molecule with the modified Glu molecule;
- and mapping the 5-hydroxymethylcytosines in the nucleic acid molecule.
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A kit comprising purified β-glucosyltransferase and a modified uridine diphosphoglucose (UDP-Glu) molecule.
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Dependencies are inferred from the claim text.