Protein isoforms for diagnosis
AssigneeOhio University
InventorsJohn J. Kopchick, Shigeru Okada, Sudha Sankaran
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Abstract
Several aspects of this invention relate to diagnosis of diabetic states in a mammal using protein isoforms. In some aspects, it relates to a method for determining the diabetic state of a mammal. This method can include, for example, (a) measuring the serum concentration of one or more protein isoforms, (b) analyzing the serum concentration of the one or more protein isoforms, and (c) determining the diabetic state of the mammal. Other aspects include kits used to perform the method. Further aspects are the isolated protein isoforms themselves, and their methods of isolation.
Claims25
A method for determining a diabetic state of a mammal comprising
- measuring a concentration of one or more protein isoforms in the mammal's plasma or serum,
- analyzing the concentration of the one or more protein isoforms, and
- determining the diabetic state of the mammal.
An isolated protein isoform selected from the group consisting of:
- Kininogen isoforms, Apolipoprotein A1 isoforms, Retinol Binding Protein 4 isoforms, Haptoglobin isoforms, and Transthyretin isoforms.
A method for isolating a protein isoform selected from the group consisting of:
- Kininogen isoforms, Apolipoprotein A1 isoforms, Retinol Binding Protein 4 isoforms, Haptoglobin isoforms, and Transthyretin isoforms comprising isolating the protein isoform using gel electrophoresis, HPLC, or LC.
A kit for the diagnosis of a stage of diabetes in a mammal comprising a composition used for the detection of a protein isoform selected from the group consisting of:
- Kininogen isoforms, Apolipoprotein A1 isoforms, Retinol Binding Protein 4 isoforms, Haptoglobin isoforms, and Transthyretin isoforms.
Dependencies are inferred from the claim text.