Optimised fermentation media
AssigneeLanzatech New Zealand Limited
InventorsChristophe Daniel Mihalcea, Jennifer Mon Yee Fung, Bakir Al-Sinawi, Phuong Loan Tran
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Abstract
The invention relates to improvements in the production of alcohols by microbial fermentation, particularly to production of alcohols by microbial fermentation of a substrate comprising CO. It more particularly relates to the provision of an inorganic organic sulfur source to a fermentation system such that one or more micro-organisms convert a substrate comprising CO to alcohols. In a particular embodiment, a microbial culture is provided with sodium polysulfide, wherein a substrate comprising CO is converted to products including ethanol and 2,3-butanediol.
Claims29
A method of improving efficiency of fermentation of a substrate including CO to produce one or more products, the method comprising:
- providing inorganic sulfur to a fermentation broth that includes one or more carboxydotrophic micro-organisms.
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A method of producing one or more products by microbial fermentation, the method comprising of:
- (a) providing a substrate comprising carbon monoxide;
- (b) in a bioreactor containing a culture of one or more micro-organisms in a liquid nutrient medium that includes one or more inorganic sulfur compounds, anaerobically fermenting the substrate to produce one or more products that include acids and/or alcohols;
- (c) capturing and recovering the one or more products produced;
- wherein the concentration of one or more inorganic sulfur compounds in the culture is maintained at a predetermined concentration or within a predetermined concentration range during at least step (b).
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A nutrient medium adapted to improve microbial growth and/or production of metabolites by one or more carboxydotrophic micro-organisms, the nutrient medium comprising one or more inorganic sulfur compounds.
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Dependencies are inferred from the claim text.