Method for detecting nucleic acid in biological sample
AssigneeOlympus Corporation
InventorYasuo Tanigami
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Abstract
The present invention is to provide a method capable of efficiently and readily detecting a nucleic acid from a biological sample such as feces. The present invention provides a method for detecting a nucleic acid in a biological sample, comprising: (A) a step of mixing a biological sample with a nucleic acid stabilizer solution comprising a water-soluble organic solvent as an active ingredient, and thereafter preserving the thus yielded suspension for a predetermined period of time; and (B) a step of performing a reverse transcription reaction and/or a nucleic acid amplification reaction with use of a part of the suspension yielded from the step (A) as a template solution, to thereby detect a nucleic acid in the suspension.
Claims15
A method for detecting a nucleic acid in a biological sample, comprising:
- (A) mixing a biological sample with a nucleic acid stabilizer solution comprising a water-soluble organic solvent as an active ingredient, and then preserving the thus yielded suspension for a predetermined period of time;
- and
- (B) performing a reverse transcription reaction and/or a nucleic acid amplification reaction with use of a part of the suspension yielded in (A) as a template solution, thereby detecting a nucleic acid in the suspension.
Dependencies are inferred from the claim text.