Primers for the rapid and specific detection of propane-oxidizing and butane-oxidizing microorganisms and methods of using same
AssigneeCal Poly Corporation
InventorsRaul Cano, Christopher Kitts, Brian Jeremy Chan
Life of this patent
Estimating the term…
Abstract
Nucleic acid sequences are provided which in an embodiment provide a primer pair. The primers are capable of amplifying a nucleic acid molecule that indicates the presence of a propane-oxidizing and/or butane-oxidizing microorganism. A method is provided which employs such primers in a process that indicates the presence of such organisms. The method is useful in detecting the presence of petroleum-like products.
Claims19
A composition of matter comprising a primer pair comprising,
- i) a first primer comprising the sequence TGGCACCGGTGG (A\G)T (C\G)TACGAIGACT (SEQ ID NO:
- 1);
- and
- ii) a second primer comprising the sequence GCGCGATCAG (C\G)GTCTT (G\C)CC (G\A) TC (SEQ ID NO:
- 2).
A method for detecting the presence of microorganisms selected from the group consisting of propane oxidizing microorganisms and butane oxidizing microorganisms in a sample, the method comprising
- a) amplifying any nucleic acid molecule in said sample with a primer pair selected from the group consisting of
- i) a first primer comprising a sequence
- (SEQ ID NO:
- 1)
- TGGCACCGGTGG(A\G)T(C\G)TACGAIGACT;
- and
- ii) a second primer comprising the sequence GCGCGATCAG (C\G)GTCTT (G\C)CC (G\A) TC (SEQ ID NO:
- 2);
- and
- b) detecting the presence of said microorganisms by determining if said primers amplify a nucleic acid molecule.
A kit comprising a primer pair selected from the group consisting of
- i) a first primer comprising the sequence
- (SEQ ID NO:
- 1)
- TGGCACCGGTGG(A\G)T(C\G)TACGAIGACT;
- and
- ii) a second primer comprising the sequence GCGCGATCAG (C\G)GTCTT (G\C)CC (G\A) TC (SEQ ID NO:
- 2).
An isolated polynucleotide primer comprising a sequence consisting essentially of a sequence selected from the group consisting of,
- i)
- (SEQ ID NO:
- 1)
- TGGCACCGGTGG(A\G)T(C\G)TACGAIGACT;
- ii)
- (SEQ ID NO:
- 2)
- GCGCGATCAG(C\G)GTCTT(G\C)CC(G\A)TC;
- and
- iii)
- (SEQ ID NO:
- 46)
- TCGAGTGGTTCGAGCACAAGTACCCGGGCTGGTA
Dependencies are inferred from the claim text.