Polypeptides having cellobiohydrolase ii activity
AssigneeDIREVO Industrial Biotechnology GmbH
InventorsNadine Koch, Oliver Kensch, Klaus Schulze-Pellengahr
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Abstract
Provided herein are improved variants of polypeptides having cellobiohydrolase II activity, nucleic acids encoding the polypeptides, vectors, host cells containing the nucleic acids and methods for producing the polypeptides. The polypeptides encompassed by this disclosure may be used in numerous applications including the use of the polypeptides for the production of biofuels and for the synthesis of platform chemicals or biopolymers from renewable sources.
Claims16
1-32. (canceled)
A polypeptide having cellobiohydrolase II activity, wherein said polypeptide comprises a substitution or deletion at a position corresponding to one or more of residues Q37, N38, Y53, S54, A65, A66 and/or H438 in CBHII from Trichoderma reesei (SEQ ID NO:
- 1).
The polypeptide of claim 1, wherein said polypeptide comprises a substitution at a position corresponding to one or more of residues Q37I, N38K, Y53(A/Q), S54V, A65P, A66Y and/or H438(S/N) in CBHII from Trichoderma reesei (SEQ ID NO:
- 1).
The polypeptide of claim 2, wherein said polypeptide consists essentially of the substitutions selected from the group consisting of:
- a) Q37I, N38K,
- b) Y53A, S54V
- c) A65P, A66Y
- d) H438S
- e) H438N
- f) Y53A, S54V, H438N
- h) Y53Q, S54V, H438S
- i) Y53Q, S54V
- j) Q37I, N38K, A65P, A66Y, H438S
- k) Q37I, N38K, Y53A, S54V, A65P, A66Y and H438S.
A polypeptide having cellobiohydrolase II activity, wherein said polypeptide having an amino acid sequence that varies from the amino acid sequence of the wild type CBHII from Trichoderma reesei (SEQ ID NO:
- 1), wherein the amino acid sequence of the polypeptide comprises at least one variation as compared with SEQ ID NO:
- 1, and wherein the at least one variation occurs at a position selected from the group consisting of positions 37, 38, 53, 54, 65, 66 and/or 438 of SEC) ID NO:
- 1, and wherein each of said at least one variations can be the seine or different and can comprise a substitution, deletion or insertion.
A polypeptide of claim 4, wherein the at least one variation comprises a variation of one, two, three, four, five, six or all seven of the positions selected from the group consisting of:
- Q37, N38, Y53, S54, A65, A66 and/or H438.
A polypeptide of claim 5, wherein the at least one variation comprises a variation selected from the group consisting of Q37I, N38K, Y53A, S54V, A65P, A66Y and/or H438S.
A polypeptide of claim 5, wherein said polypeptide consists essentially of the substitutions selected from the group consisting of:
- a) Q37I, N38K,
- b) Y53A, S54V
- c) A65P, A66Y
- d) H438S
- e) H438N
- f) Y53A, S54V, H438N
- h) Y53Q, S54V, H438S
- i) Y53Q, S54V
- j) Q37I, N38K, A65P, A66Y, H438S
- k) Q37I, N38K, Y53A, S54V, A65P, A66Y and H438S.
A polypeptide which has at least a minimum percent sequence identity and/or percent homology to the polypeptide of claim 1, wherein the minimum percent identity and/or homology is selected from the group consisting of at least 50%, at least 60%, at least 75%, at least 80%, at least 85%, at least 90%, at least 93%, at least 95%, at least 96%, at least 97%, at least 98%, and at least 99%.
A nucleic acid encoding a polypeptide of claim 1.
A host cell comprising the nucleic acid of claim 9.
An enzyme composition comprising at least one polypeptide of claim 1.
A method of producing a polypeptide having cellobiohydrolase II activity comprising the steps of:
- (a) culturing the host cell according to claim 10 in a suitable culture medium under suitable conditions to produce polypeptides having cellobiohydrolase II activity;
- (b) obtaining said produced polypeptides.
A feed additive comprising at least one polypeptide of claim 1.
A method of converting biomass to sugars comprising contacting said biomass with at least one polypeptide of claim 1.
A method of alcohol fermentation comprising the use of at least one polypeptide of claim 1.
Dependencies are inferred from the claim text.