Stabilization Of Perhydrolases
AssigneeE. I. Du Pont de Nemours and Company
InventorsRobert DICOSIMO, Arie Ben-Bassat, Mark S. Payne, Raymond Richard Zolandz
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Abstract
Disclosed herein is a method for stabilization of the perhydrolase activity of the CE-7 esterase in a formulation with a carboxylic acid ester that employs the addition of a buffering agent, substantially undissolved, to the mixture of the CE-7 esterase and the carboxylic acid ester. Further, disinfectant and laundry care formulations comprising the peracids produced by the processes described herein are provided.
Claims21
1-17. (canceled)
A formulation used as a first component in a multi-component peracid generation system, said formulation comprising a mixture of:
- (a) at least one carboxylic acid ester selected from the group consisting of monoacetin, diacetin, triacetin, monopropionin, dipropionin, tripropionin, monobutyrin, dibutyrin, tributyrin, and mixtures thereof;
- (b) an enzyme powder comprising
- (i) at least one enzyme structurally classified as a CE-7 enzyme and having perhydrolysis activity and a signature motif comprising:
- (A) an RGQ motif at amino acid residues 118-120,
- (B) a GXSQG motif at amino acid residues 179-183, and
- (C) an HE motif at amino acid residues 298-299
- when aligned to reference sequence SEQ ID NO:1 using CLUSTALW,
- (ii) at least one excipient, and
- (iii) optionally at least one surfactant;
- and
- (c) at least one buffer;
- wherein said at least one buffer enhances the stability of said at least one enzyme when present in said formulation as compared to the perhydrolase activity of said at least one enzyme when the at least one excipient is not present.
22-26. (canceled)
Dependencies are inferred from the claim text.