Death Receptor CD95 Controls Neurogenesis of Adult Neural Stem Cells in Vivo and in Vitro
AssigneeDeutsches Krebsforschungszentrum
InventorsAna Martin-Villalba, Nina Corsini, Elisabeth Letellier, Susanne Kleber, Stefan Klussmann
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Abstract
The present invention relates to the activation of the CD95L/CD95 system for inducing neuronal differentiation in vitro and in vivo and the use of CD95L compositions to effect differentiation.
Claims20
Use of a composition comprising an activator of the CD95-Ligand (CD95L)/CD95-Receptor (CD95) system for the manufacture of a medicament for the treatment of a neuronal injury.
The use of a composition comprising a polynucleotide encoding CD95L for the manufacture of a medicament for the treatment of a neuronal disease or a neuronal injury.
A method for treating injured neuronal tissue comprising:
- (i) treating stem cells with an activator of the CD95-Ligand (CD95L), CD95-Receptor (CD95) system, and
- (ii) introducing those treated stem cells to the site of neuronal injury, wherein the treated stem cells differentiate into new neuronal cells at the site of injury.
A method for treating injured neuronal tissue comprising:
- (i) engineering a stem cell to express a polynucleotide encoding CD95L;
- and
- (ii) introducing that engineered stem cell into a subject, wherein:
- (i) the stem cell migrates to the site of injury;
- and
- (ii) (ii) the polynucleotide is operably linked to regulatory elements that are preferably only active in the tissue that has been injured.
A method for treating injured neuronal tissue comprising introducing a viral vector into a subject, wherein the vector comprises a polynucleotide that encodes CD95L and is operably linked to regulatory elements that are preferably only active in the tissue that has been injured.
A vector comprising a polynucleotide that encodes a CD95L protein, wherein the polynucleotide is operably linked to:
- (i) a promoter that is preferably active in neuronal tissue only and
- (ii) a terminator functional in neuronal tissue.
A stem cell, which comprises in its genome a non-naturally occurring polynucleotide that comprises a polynucleotide that encodes a CD95L protein, wherein the polynucleotide is operably linked to:
- (i) a promoter that is preferably active in neuronal tissue only, and
- (ii) a terminator functional in neuronal tissue.
Dependencies are inferred from the claim text.